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The sample combination being divided and analyzed is introduced, inside a discrete smaller volume (ordinarily microliters), into your stream of cell section percolating in the column. The components from the sample transfer with the column at various velocities, that are a purpose of certain physical interactions Along with the adsorbent (also call

-hydroxybenzoic acid—on the nonpolar C18 column employing a aqueous mobile period that features a buffer of acetic acid and sodium acetate. Retention moments are shorter for less acidic cell phases because Each individual solute is existing in an anionic, weak base form which is a lot less soluble from the nonpolar stationary stage.This posed a p